By Anonymous on Friday, October 3, 2003 - 04:23 am:
which is the better detector for compound that havining low absorbance
By R.C. on Friday, October 3, 2003 - 06:37 am:
Hmmm... that's a very broad question you've given us here. Some thoughts:
Does it have low absorbance at all possible wavelengths? You may be able to use an absorbance detector if you can find a wavelength compatible with your eluent.
Is there a published method for pre-derivitization or post-column derivitization to give it a chromaphore?
Does it fluoresce? Many organic compounds fluoresce. You can use a fluorescence detector in that case.
If your method is isocratic, you can use a refractive index detector. This is commonly used for alcohols and sugars. There are also evaporative light scattering detectors
For nitrogen or sulfur containing organic compounds, there are CLND and CFSD detectors, respectively.
And there's always LC-MS.
By Anonymous on Friday, October 3, 2003 - 07:40 am:
Hi, R.C.,
Could you please explain me what CFSD is? Thanks!
By R.C. on Friday, October 3, 2003 - 11:03 am:
CLND - chemical luminescence nitrogen detector, detailed in recent edition of LC-GC, pg 376 VOLUME 21 NUMBER 4 APRIL 2003
CFSD - chemical fluorescence sulfur detector, I only just heard of it, I don't know exactly how it works, 'tho it is probably similar to CLND
By Anonymous on Monday, October 6, 2003 - 03:25 am: