By Anonymous on Monday, October 6, 2003 - 06:11 am:
Can anyone help me with a mobilephase that can seperate D3 and E vitamin. I am using ACN:MeoH:H2O
70:20:10, but the seperation is not good enough.
Regards
Kim
By Durk on Tuesday, October 7, 2003 - 07:13 am:
Hi Kim,
We have a HPLC method for quantifying vitamin A, A-palmitate, A-acetate, D2, D3, E, E-acetate. We use a Zorbax Eclipse XDB-C8 column and a mobile phase gradient of water and methanol. With this method vitamin D2 and D3 can not be separated.
Durk
By Anonymous on Friday, October 10, 2003 - 09:43 am:
You may want to look at the Alltech Prevail C18 literature. It looks like they are using 75% ACN 25% MeOH with their column and it seems to separate fine. www.alltechweb.com