By valentine wascotte on Friday, October 10, 2003 - 03:03 am:
I'm looking for a very sensitive method (nM) for creatinine dosages with maximum 1 ml sample.
I'm afraid about creatine interference.
Thank you for your help.
By A.Nonymous on Friday, October 10, 2003 - 08:05 am:
I have found this with a litle bit searching:
Column:XPERBOND C18, 125Å, 10µm, 300mm x 3.9mm
Mobile Phase: 1.44g of dodecyl sulfate sodium in 1000mL of a mixture of acetonitrile, water and phosphoric acid. (300:700:1)
Flow Rate: 1.0mL/min
Detector: 210nm
Although I don't know Xperbond C18, I guess it can be done with another C18 column. Try to vary your dodecylsulfate and acetonitrile, and you will find your method.