Does anybody know of any good applications book / literature with wide coverage for HPLC methods for different kind of compounds

There is collection by Lunn. It is expensive, but covers a lot. Look at Amazon.com!
Alternatively the websites of major column maunfaturers have a ton of application examples.

Any hplc method development book is there online which is free of cost or effective tools for the method development free of cost not the product and boruch from suppliers

There is nothing wrong with the applications literature of column manufacturers. We try to give good advice on a range of subjects, from solid applications support to advantages of our products for different application areas. In some cases, our applications chemists belong to the best in their respective fields. In our lab, we have developed effective methods development strategies for HPLC methods or sample preparation. I would not throw away such high-class advice just because it comes from a column manufacturer.

Why would you expect valuable information like this to be free? The authors of these books have spent many years learning the art and science and deserve to be compensated.

Hi Uwe Neue and all,

Definitely I would not mind buying a good book /application collection. In the meanwhile if anybody has come across with an application for HPLC analysis of an acid chloride such as a substituted benzoyl chloride (if possible w/o derivatisation) could you share the same.

Such an application would have to avoid all contact with hydroxyls or other functional groups that will react with an acid chloride, since even the air and everything in it contains water or has a surface coated with water, thus this is difficult indeed.

But if you react the acid chloride with an anhydrous alcohol in anhydrous ether (overnight reaction at 37°C) then you will be able to determine how much carboxylic acid is present in the acid chloride.

That value is a critical requirement of any application for purity measurement of an acid chloride.

Thanks for your feedback. I will try it out

To anon 031013 - The suppliers offer this information simply to earn money. They sell columns and a customer is more likely to buy a column when his application runs on it.
To venksin - There might be good books around. However, the number of different organic compounds is enormous and so are the possibilities for separation. Look onto the vendors application libraries! They are quite usefull. I have listed some on www.abuske.claranet.de
If you are working with comercially available substances ask again the suppliers, they should have QC methods. Or search publications on the substance. For benzoyl chloride and "degradation product" look at http://www.alltechweb.com/literature/catalog500/434.pdf


Alex

Try literature search through Chemical Abstratcs etc.

A. Buske: There are some dead links in the text of your main page (404 errors), but the equivalent links at the top of the page seem to work. Nice page, though, thanks!

Hi Buske and all,

thanks for the link.
On acid chloride of different options tried I found derivatisation with an amine (morpholine, aniline etc) works better. The derivatisation happens in presence of a base such as KOH. The acid remains free.

Thanks for all suggestions

You All are correct that we have to Support the authours for there learning and efforts to make a great book , but you know this world is governed by the UK and US people so as its not always the book cost willbe in dollars and pounds and its always bea 100 to 150 dollars , or sometimes many more goes up to 300 dollars .

so you consider a person from asian region if he has to buy book lets say indian he has to spend around 100 x 50 = 5000 rs which is a quite big amout as in india any study bo0k does not cost more than 1000 rs .

so if the cost is in pounds he has to pay around 100 x 65 =6500 which is still very high , so people must be looking for free articles and books on net so they can download and read for a very cheap and it will be the globlisation , and this is the region why the people from US, UK are masters in HPLC and related chromatographic techniques even pioneers companies inthis field are from this region , and people from asian region are quite behind , i visited this place several times and we have to teach the people a typical silica chemistry which they suppose to know when they starts there job in this field this is the reason of governance of US And UK people in this field and there are many field like this which are not know to we chromatographers .


but i really appriciate the afforts of chromforum m0derators and its founders to provide such a wonderful and dream discussion forum to discuss and learn the amazing things about the hplc and related techniques and all types of chromatography.