I want to do quantitative analysis of the following substances in biological samples, using RI detection: ethanol, glycerol, xylitol, arabitol, glucose, Arabinose, xylose. Does anybody know an alternative to ligand exchange/ion exclusion phases that are more robust towards sample matrix?

I use total cell extracts and fermentation broths.Are there cheap and easy alternatives to SPE/filtration (maybe recipitation + centrifugation),and which compounds besides proteins do You regard as critical, especially towards PS/DVB based phases?

In my opinion, SPE is nearly ideal. On a reversed-phase sample preparation packing like Oasis, the sugars will break through unretained and all the junk will remain.

You can try HILIC as an alternative to the ligand-exchange size-exclusion stuff. A silica column like Atlantis HILIC will do fine. I don't remember, if all your peaks of interest are separated or not.

Reversed phase SPE would be ideal for this! I've used a reversed phase packing for sample prep, which holds up all the hydrophobic goop, then ion-exclusion for analysis of the aqueous eluate, which works fine. HILIC would work well too and probably give better resolution. Detection via RI or ELSD would be easiest.

Good Luck!

Chris

A viable option is to separate your compounds of interest via high pH anion exchange with amperometric detection. The high sensitivity of the detection method allows sample prep to be fairly minimal (centrifuging the sample and then simple solution). Details of the protocol for application to fermentation broths can be found that the following link: http://www.dionex.com/servletwl1/FileDownloader/slot114/8933/AN122.PDF

i want to analyse souluble sugar with GC. Would you please help me to find the best procedure for extracting the sugar and purifying it. I want do this analyse on matthiola incana. thanks