By Anonymous on Thursday, October 16, 2003 - 06:45 am:
Does anyone have a 'recipe' for 2,4-DNP for aldehyde analysis by HPLC. Every time I see one it seems to be different.
Thanks in anticipation
John T
By Been there on Thursday, October 16, 2003 - 10:51 am:
Years ago, we used Prepare by dissolving 0.62 g DNPH in 250 ml 6N HCl. Lately, we've been using
DNPH reagent (0.12 % 2,4-dinitrophenylhydrazine in HPLC acetonitrile): weigh 0.11 – 0.13g
2,4-dinitrophenylhydrazine (to the nearest 0.01g) into a 100 ml volumetric flask; mix and dilute to volume with acetonitrile (mix well to dissolve) Make fresh weekly. James R. Dahlgren and Melvin N. Jameson, “Determination of Formaldehyde and Other Aldehydes in Industrial Surfactants by Liquid Chromatographic Separation of Their Respective 2,4-Dinitrophenyl-hydrazone Derivatives,” Journal of the Association of Official Analytical Chemists (Vol. 71, No. 3, 1988), pp. 560-563.
By Einar Pontén - SeQuant AB on Thursday, October 16, 2003 - 02:51 pm:
I recommend dansylhydrazine if you are not stuck to DNP for the analysis.
We have developed a flexible and easy to use product for dansylation of aldehydes and ketones.
http://www.sequant.com/products/sampleprep/2200.shtml
By Anonymous on Friday, October 17, 2003 - 12:59 am:
Thank you both for that information.
John
By Anonymous on Wednesday, November 12, 2003 - 08:53 am:
Instead of DNPH: Does anyone have a recipe for lutidine reaction with aldehydes > formaldehyde ?
By Einar Pontén - SeQuant AB on Friday, November 14, 2003 - 01:28 pm:
We still have an offer for free evaluation samples!
It is remarkable that the chemists in Asia realise the merits of danzylhydrizine while other s don't.
Explanations?