By andreasg on Wednesday, October 22, 2003 - 07:44 am:
I need HPLC-Methods to characterise the extract of hamamelis v.L. (gallic acid, hamameltannine, cyanidine, catechine, flavones
By Einar Pontén - SeQuant AB on Wednesday, October 22, 2003 - 04:22 pm:
HILIC, hydrophilic interaction liquid chromatography is likely a suitable technique for these hydrophilic and polar compounds.
It is certainly worth a try.
By andreasg on Thursday, October 23, 2003 - 07:19 am:
thanks for this tip, is their any literature avialable????
By Einar Pontén on Thursday, October 23, 2003 - 11:52 am:
A brief introduction is available at:
http://www.sequant.com/pdf/SeQuant.ZIC-HILIC.column.pdf
Send me an e-mail if youi would like to get a list of refs.
By A.Mouse on Thursday, October 23, 2003 - 06:09 pm:
Before I would go to HILIC, I would satisfy myself first that standard reverse-phase HPLC won't work. Run a standard pH-gradient with acetonitrile and a collection of standards of your compounds at acitic pH, maybe with phosphate at pH 2, unless you want to use MS. All these compounds will work well on a reversed-phase column, maybe even better on one of those EPG phases or Atlantis C18. Only if you do not get retention of your compounds (unlikely!). I would recommedn to go to HILIC.