By Waqar Shahid on Wednesday, October 22, 2003 - 11:41 pm:
Dear, Listener.. please help
i ws doing HPLC pump calibration.when i occured a problem.the claibration ws of pump's gradients.
I prepared a .3 Aceone solution with water. and degas water for mobile phase.the reading for it in detector is very low.The calibration process indicates it sould be .5 or .7 but i reading comes like .0035 to .0044.this variation is change in the amount of acetone in solution.My question is .i think the pump is working fine.The problem is with detector.what should i do? should i do should i increase the amount of accetone or should i align the detector.Remember The detector is aligned and is working fine in other condition.
Please advice
a USER
By Anonymous on Thursday, October 23, 2003 - 03:25 pm:
Wavelength?
By Anonymous on Friday, October 24, 2003 - 05:14 am:
Try running it at 265nm. That is about the max wavelength for acetone.
By MG on Friday, October 24, 2003 - 06:00 am:
Wagar, we have run this test at 210 nm with 1% acetone in bottle B. When it has failed, the problem has always been pump related. I think that even 0.3% acetone should provide sufficient absorbance at 210 nm, if that is your wavelength.
By R.C. on Friday, October 24, 2003 - 06:47 am:
When one of our pumps failed a post preventive maintenance calibration, the technician questioned the quality of the acetone. I figure he was just making excuses, but it may be something else to check. 1% acetone will usually completely saturate the Waters prep-scale detectors we have. We usually use 0.1 % to 0.5 % acetone.