By Anonymous on Friday, November 7, 2003 - 11:44 am:
Hello Users,
im looking for an method to determinate salvinorin (divinorin in salvia divinorum (the sage). Have anybody a rapid method to quantificate this substance in salvia divinorum??? i am also looking for lc/ms methods..... thanks a lot
By Zelechonok on Saturday, November 8, 2003 - 07:36 am:
See: Gruber, John W... High performance liquid chromatographic quantification of salvinorin A from tissues of Salvia divinorum Epling & Jativa-M. Phytochemical Analysis (1999), 10(1), 22-25
Salvinorin is quite hydrophobic and has no ionizable groups, so any reverse phase column with high concentration of organic 50-70% in the mobile phase will give a good method. Compound is UV active too. Normal phase chromatography can be used as well with hexane/alcohol. All methods are MS compatible. Ionization can be a problem though.