By jpersoon on Tuesday, November 18, 2003 - 06:56 am:
How do I pack a HPLC column, without buing an expensieve slurry
packing pump. The column has to be 150 mm long 4.6 mm diameter. The
stationary phase will be in the first place silica beads (superspher,
Merck, 100 µm, 60 A pores) later it is going to be a new kind of
zeolite.
THX
By Chris Pohl on Tuesday, November 18, 2003 - 09:55 am:
As a bare minimum you can pack a column using a standard HPLC pump at 10 ml/min and coupling your column to be packed to a slurry reservoir (preferably a 7.6mm column body using a reducing union). Not sure what a good slurry solvent is for bare silica but you might try acetone.
By Uwe Neue on Wednesday, November 19, 2003 - 07:34 pm:
This particle size can be dry packed. Pour small amounts of packing into the column, then tap it on the table until it stops moving (or, since you may not see it, for about 1 to 2 minutes), then add some more stuff and repeat the same thing until the column is full... Haven't encountered this in thirty years...