By Anonymous on Tuesday, November 18, 2003 - 09:12 am:
I get ghostpeaks if I just inject the eluent. I get a 2 min wide peak (about 2 mn after injection)without a column connected.
I changed some parts in the Rheodyne valve (8125) but it got worse (2 peaks instead of 1).
Also the pressure drops every time I inject up to 80 psi.
I am thankful for every suggestion.
By shital pathak on Tuesday, November 18, 2003 - 01:06 pm:
I think Changing the purge(injector wash)solution and washing of injector should solve the problem.
By Anonymous on Tuesday, November 18, 2003 - 05:34 pm:
What is mobile phase?
By Anonymous on Tuesday, November 18, 2003 - 11:48 pm:
Is it possible that air enters your system when you inject?
By Anonymous on Wednesday, November 19, 2003 - 12:54 am:
The mobile phase is 0.05% TFA in water. And I am using a capLC with flow rate of 2.5 uL/min.
I do think that air enters the system when I inject. The last time I tried, I got air spikes with the second peak.
Also, if I wash the sample loop there is air coming out. And I am sure I donīt put any air in with the syringe. But I donīt know if this happened recently or if it always did that because i never checked that before.
By zelechonok on Wednesday, November 19, 2003 - 08:25 pm:
If you degas your mobile phase and not degas your sample you can see the air dissolved in your sample as a broad peak like you described.
If you bypass degasser (you can do it with isocratic method) this peak should disappear.