By Anonymous on Tuesday, September 28, 1999 - 11:50 am:
I am using Millenium32 system and I would like recommendations on how to calibrate the instrument.
We are running 14 samples
with 3-5 standards
I am interested in the bracketing technique.
Also are there any sites which explain GLP procedures in reference to calibration
By A.A. on Wednesday, September 29, 1999 - 05:31 am:
Are you looking for "How To" information (example: How do I make Millennium automatically make calibration curves) or a recommendation on procedures (example: 3 standards are not enough)?
A.A.
By MJ on Wednesday, September 29, 1999 - 12:29 pm:
in general how about a website that references how to calibrate any brand HPLC In reference to GLP?
By stepluke on Thursday, September 30, 1999 - 08:54 am:
Hello
Try
{http://www.labcompliance.com/,http://www.labcompliance.com/}
Cheers
By Anonymous on Thursday, September 30, 1999 - 11:10 am:
A recommendation on procedures. I know how to create a calibration curve using millenium. I just want to make sure I am calibrating enough during the run to account for changes during the run.
By Anonymous on Thursday, September 30, 1999 - 12:44 pm:
Try checking some of the documents here:
http://www.fda.gov/cder/guidance
You may be able to find the answer to your question.
By Anonymous on Thursday, September 30, 1999 - 12:56 pm:
OK
It sounds like you just want someone to say "Use this number of standards, over this range of concentrations, and run before and/or during and/or after your samples." It is a hard thing to say because every situation and anaylsis is different. You have written 'I just want to make sure I am calibrating enough during the run to account for changes during the run'. What changes are you talking about? Your 3 stds before the 14 sample may be more that adequate for your analysis. A little more info might be helpfull.
By debm on Wednesday, October 6, 1999 - 08:21 am:
We run a standard curve up front. Then our procedure is to bracket a midpoint standard of the standard curve every six injections and one at the end of the run. This is a procedure written into our validated methods. Hope this helps.
By LaurenceB on Friday, October 8, 1999 - 03:57 am:
Working in a contract research lab under GLP in the UK, we used to inject 5 standards to start with, then our middle standard every two or three injections and then the five standards at the end. A bit of an overkill, but it didn't feel so at the time. It also brought out inconsistent injection volumes from some of our equipment a few time!
Posting is currently disabled in this topic. Contact your discussion moderator for more information.