By Maya on Friday, December 5, 2003 - 01:44 am:
Is it possible to run an analysis of food colours mixture (tartrazine, amaranth, brilliant blue) using UV-VIS detector with deuterium lamp?
By Anonymous on Friday, December 5, 2003 - 01:48 am:
Try looking at Agilent Pub.No12-5965-5124E which is HPLC for Food Analysis. You may need a diode array for id.
David
By Consumer Products Guy on Friday, December 5, 2003 - 06:46 am:
We've done a few FD&C colors in low ppm range using HPLC with a UV detector in the visible range. We used an Agilent 1100 DAD detector, and feel one gets better sensitivity (1) in visible range, less background (2) when using a detector with a separate tungsten or similar lamp rather than a single all-purpose deuterium lamp. Many dyes are sulfonic acid salts so appropriate mobile phase such as 0.01 M perchlorate is necessary. Also see L. Gagliardi, G. Cavazzutti, A. Amato, A. Basili, and D. Tonelli, “Identification of Cosmetic Dyes by
Ion-Pair Reversed-Phase High-Performance Liquid Chromatography,” Journal of Chromatography,
394 (1987), pp. 345-352.
By Maya on Tuesday, December 9, 2003 - 01:47 am: