By Anonymous on Thursday, December 11, 2003 - 11:44 am:
Hi,
I have a question but because I just started working last month I do not want to bug my boss or co-wokers. What is "direct plazma injection" in several articles I read people have different procedure/approach. Does it involve any sample treatment or not? If yes what is it?
By Uwe Neue on Thursday, December 11, 2003 - 12:33 pm:
Plasma is the liquid part of blood, i.e. without the platelets. Plasma injection is the injection of this stuff onto a column without attempting to remove the plasma proteins, i.e. without sample preparation. You still may want to adjust the pH to break the interaction of your drug of interest with the plasma proteins, and you will need to add an internal standard. Of course, you need a column with which you can do this...
By Anonymous on Friday, December 12, 2003 - 06:07 am:
Thank you Uwe.
Nick T.
By OMARIKHALID on Wednesday, April 28, 2004 - 06:31 am:
WHEN WE TAKE A BLOOD IN EDTA-TUBE (ANTICOAGULANT-TUBE) THEN CNTRIFUGE THE TUBE LET SAY AT 3000 ROUND/MINUTE YOU WILL GET 2 PHASES THE UPPER ONE WHICH IS YELLOW (PLASMA) AFTER SEPARATE THE PLASMA AND WITHOUT MAKING ANY TREATMENT LIKE EXTRACTION (I.E LET PLASMA AS IT IS) AND INJECT IT DIRECTLY VIA INJECTION PORT TO THE COLUMN THIS PROCESS CALLED "DIRECT PLASMA INJECTION"