By Anonymous on Friday, January 30, 2004 - 01:13 pm:
Fellow chromatographers:
I am trying to determine TRIS (tromethamine) in a pharmaceutical formulation. Can anyone give me some direction (column, mobile phase, detector, etc.)? I'm thinking RI detection because no chromophore but not sure. Thanks for any advice.
-Jeff
By Uwe Neue on Saturday, January 31, 2004 - 12:11 pm:
This is an ideal application for HILIC - i.e. a silica column with 70 to 80% acetonitrile and water, maybe with some buffer. It is not impossible that you see the compound in the low UV. I would try this first before using an RI, since it takes half a century for the baseline to settle in the RI, when you change the mobile phase.
By AllsepTech on Monday, February 2, 2004 - 10:54 am:
Another alternative to HILIC can be mixed mode chromatography. TRIS can be analyzed same way as ethanolamines with ELSD detector. See example at our website
http://allsep.com/makeChr.php?chr=Chr_041
By Anonymous on Monday, February 2, 2004 - 01:28 pm:
Thanks for the advice folks,
I'll try Uwe Neue's setup first as I don't have ready access to an ELSD detector. I'll keep you all posted.
-Jeff