By Jesus on Saturday, January 31, 2004 - 12:27 am:
Hi, I'm a student and I have to answer a question about gas chromatography.
They gave me a mixture of sugars from the cell wall and I did a gas chromatography. in the results I observed two separeted group of peaks:
The first group of sugars coming out in the graphic are ramnose, fucose, arabinose and xilose
Separated from them by 4 minutes the second group shows manose, galactose, glucose and inositol (that we used as within standart)
The question is why can I identify two groups?
Thanks and I apologised for my bad english.
By Anonymous on Monday, February 2, 2004 - 09:33 am:
Jesus,
Look at the structures of the sugars.
These are 5 and 6 carbon sugars but they are not separated by the number of carbons in each. Instead it is the number of free hydroxy functional groups.
The first group has 4 hydroxy groups and the second has 5 hydrxy groups per molecule.