By Anonymous on Wednesday, May 19, 1999 - 09:51 am:
c18 column, 3%acn, 0.15Et3N, 0.08%H3PO4,0.12%B8
uv detection. The problem: the solvent elutes at the same retention time as the glucosamine. Different mobile phases and columns tried.
By Chris Pohl on Thursday, July 1, 1999 - 06:08 pm:
I need more information. What, is B8? Is this your abbreviation for borate? If so, then it appears that you are attempting to separate glucosamine by ion-pairing. I would switch to a more potent ion pair reagent such as tetrabutylammonium phosphate. Of course,the best way to analyze this compound is by anion exchange with electrochemical detection. Is this option out?
By claudiu on Wednesday, March 21, 2001 - 09:06 am:
I have a question. It is possible to use UV detection for glucosamine ? In such case, which is the wavelenght you have used ?
By shirish on Tuesday, June 26, 2001 - 09:44 pm:
glucosamine can be analysed by the derivatised uv hplc it can be derivatised with the phenylisothicyanate and then uv detection method is quite simple you have to derivatize ation for about 16 hours in phosphate buffer ph 8.3 and chromatographic system is like this
bondapack c18
flow rate 1.2 ml/min
wavelength 254
mp methanol: water: hac(69;21;01)
ok if you know any other hplc or uv method then inform to me at shi_ri@usa.net
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