By s. board on Friday, October 15, 1999 - 09:10 am:
I'm wondering if you can quantitate 20% ethanol by HPLC with an RI detector.
By fin on Friday, October 15, 1999 - 10:54 am:
why HPLC ? woud gc not be more suiteble?
By Anonymous on Friday, October 15, 1999 - 01:52 pm:
A GC would be the obvious method of choice... however, if you have an HPLC and want to use it, it should work. I think you will have to go normal phase to get any retention. A carbohydrate column with low organic may also work but then the RI detection will be iffy. Your ethanol peak will probably be a negative peak since the RI is lower than for most organic solvents.
By Tom Jupille on Saturday, October 16, 1999 - 09:52 am:
Ethanol can be analyzed by HPLC using an ion exclusion column (yes, I know ethanol is non-ionic, but it is retained on this type of column). I know that BioRad can provide a procedure on their column. You might check with Dionex or Hamilton also.
-- Tom Jupille / LC Resources
By Rachel on Wednesday, October 20, 1999 - 03:08 am:
In response to Tom,
A Nucleosil 300 OA does the job nicely. Machery-Nagel have an application for the analysis of sugars / acids / alcohols in their applications book - number 246.
You can also do the analysis on a polymeric carbohydrate column such as ThermoQuest / Hypersil's HyperREZ Carbohydrate H+ using 2.5mM H2SO4 as the eluent and RI detection.
By Anonymous on Friday, October 22, 1999 - 07:17 am:
Hypersil shows separations on either their HyperREZ carbohydrate 100 Ag+ column or their hyper REZ rapid alcohols column.
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