Mobile Phase Question

Chromatography Forum: LC Archives: Mobile Phase Question
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Brent on Monday, March 22, 2004 - 06:29 am:

Most Reverse Phase columns are not stable with basic mobile phases. Is that true for hydrophobic bases as well? Can you use a mobile phase that is basic if it is anhydrous?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Uwe Neue on Monday, March 22, 2004 - 03:35 pm:

It is the OH- ions that attack the bonded phase or more precisely the silica. In order to get OH- ions, you need water


Top of pagePrevious messageNext messageBottom of pageLink to this message  By A.Mouse on Saturday, March 27, 2004 - 10:44 pm:

Uwe
Does this mean that one can use triethylamine as a mobile phase for bonded phases? I never considered this, and I have not seen this anywhere either.


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