1. what is the advantage of methanol then ACN (acetonitril)? why we choose methanol as mobile phase?
2. to get a good resolution and the good peak, what kind of thing must we up grade for a good peak and good resolution?
3.what is the function of internal standard? and when we used the internal standard?
4,how is of the kind of buffer effect on chromatogram profile?
5. how is the ph effect on chromatogram profile?
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By tom jupille on Thursday, March 25, 2004 - 10:36 am:
We have a free on-line course which covers the answers to most of your questions:
http://www.lcresources.com/resources/reslinksform.html
By the way, your english is quite understandable