By Anonymous on Friday, March 26, 2004 - 01:49 am:
I just want to know how to prepare the pancreas tissues sample for polyamine HPLC measurement . And how to store the samples , storage conditions like temperature and so on . Any help will be appreciated !
By Anonymous on Friday, March 26, 2004 - 01:54 am:
I just want to know how to prepare the pancreas tissues sample for polyamine HPLC measurement . And how to store the samples , storage conditions like temperature and so on . Any help will be appreciated !
By RH on Tuesday, March 30, 2004 - 02:43 am:
I would store biological material frozen (best -80°C) and do all preps on ice. Biologically interesting polyamines can usually be extracted in buffer, so You just need to use a homogenizer (potter, bead mill, grinder etc.).
There are many possibilities for HPLC-analysis of polyamines, e.g. Price et al.(1992):J. Chromatogr. 598:91ff.
By Anonymous on Thursday, April 1, 2004 - 12:52 am: