By Anonymous on Monday, March 29, 2004 - 07:28 am:
Can anyone tell me whether or not you should condition new columns, Reverse or Normal Phase, before they are used for testing? Many thanks.
By Anonymous on Monday, March 29, 2004 - 10:35 am:
read the manual that comes with the column.
By Uwe Neue on Monday, March 29, 2004 - 04:28 pm:
You always need to equilibrate with your mobile phase. Depending on your mobile phase and the column, this can be very fast, or it may take a long time. Most of the time, some 20 to 30 column volumes are enough. In general, there is no reason for doing anything else.
By DelphineG on Tuesday, March 30, 2004 - 06:55 am:
I'd like to add that you should check for compatibility between the solvent storage of the column at delivery and your mobile phase!!!! An intermediary step could be needed if they are not compatible.