By Rajini on Tuesday, October 19, 1999 - 08:28 am:
Help! Please suggest a HPLC/GC method for Vitamin D in Shark liver oil (anything other than that specified in the USP).
By Thierry on Wednesday, October 20, 1999 - 02:54 am:
Dear Rajini,
Use SPE to extract the Vitamin D from the shark oil then use a 5 micron 250 x 4.6mm HyPurity Elite C18 (Hypersil)column or similar (Kromasil)under the following conditions :
Eluent : Methanol : Acetonitrile 30 : 70
Flow Rate : 1.0 ml/min
Detection : UV 254nm
Temperature : 40 degrees Celsius
Vitamin D2 elutes at 12.69 minutes
Vitamin D3 elutes at 13.40 minutes
All the best and goog luck with the chromatography.
By Rajini on Tuesday, October 26, 1999 - 10:36 am:
Dear Thierry
Thank you very much. Do you know of any references for solid phase extraction? I haven't used it in the past.
By Wilhelm Leung on Friday, October 29, 1999 - 06:44 pm:
Check out the Bakerbond or Varian Bond-elut SPE application notes or their bibliography. Or you can call their technical support for help.
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