By Andy Jones on Wednesday, October 20, 1999 - 02:46 am:
Can anyone tell me how I can calculate this from the percentage carbon of the media ?
Thanks
By Tom Jupille on Wednesday, October 20, 1999 - 06:15 am:
Unfortunately, you can't!
Depends on the type of exchanger and degree of derivatization. It's quite possible to have two columns with similar %C but quite different amounts of charge.
By Andy Jones on Wednesday, October 20, 1999 - 07:58 am:
Thanks.
Have you got any idea how I can calculate this figure ?
By Tom Jupille on Thursday, October 21, 1999 - 07:23 pm:
I'm not sure about calculating it, but it can be *measured*. Simply load the column with an ion you can measure (ammonium?) and then displace it quantitatively with a more strongly bound ion (potassium?), then measure the amount of the first ion displaced (copper complex / colorimetry?). Check with Bio Rad; they used to have tech notes on a lot of this kind of stuff. Maybe someone else can come up with a specific reference?
-- Tom Jupille / LC Resources
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