By Anonymous on Monday, April 19, 2004 - 05:54 am:
Does anyone know of a good RP HPLC condition for azide analysis. I have seen a lot of IC conditions, but very few RP HPLC.
Let me know.
RK
By Anonymous on Monday, April 19, 2004 - 10:51 am:
do you have acces to gc? i've done azide with gc-ms after derivatisation with pentafluorobenzylbromide.
By Anonymous on Monday, April 19, 2004 - 01:53 pm:
Yes, I do. But we would prefer HPLC method.
Let me know.
RK
By Anonymous on Monday, April 19, 2004 - 01:55 pm:
Azide is ionic (N3-) and would require Ion Pairing in RP HPLC. Any comments?
RK
By A.Mouse on Monday, April 19, 2004 - 05:20 pm:
I would look first for ion-exchange or ion chromatography methods. I bet that there is a method in ion chromatography. If neither is desired, I would think that the development of an ion-pairing RP method is not impossible either. But this is work, and I prefer not to have to work.
By Anonymous on Tuesday, April 20, 2004 - 05:22 am:
Yes, there are several Ion chromatographic methods with conductivity detection. We actually have one, which has a lot of problem (baseline drift, and ghost peaks etc).
By Anonymous on Tuesday, April 20, 2004 - 08:34 am:
what about coulorimetric method by spectrophotometer at 462 nm after reaction with ferric chloride at acidic conditions.
By MG on Tuesday, April 20, 2004 - 01:54 pm:
If there is enough hydrophobic character on the molecule, I think it would be worth a try on RP without ion pairing. Just make sure you use a buffer.
By Chris Pohl on Tuesday, April 20, 2004 - 04:51 pm:
Considering the fact that you have had problems with baseline drift and ghost peaks, perhaps your problem is related to the specifics of your method. Ion chromatography is generally the preferred methodology for azide. What column are you using for the separation? What eluent system are you using? What type of suppressor are you using? If you give me a bit more information, probably I can identify the areas responsible for your performance problems.