By HUANG on Tuesday, April 20, 2004 - 11:57 pm:
Would anyone know of an HPLC method for analysing 3-aminopyrazole-4-carbonitrile, C4H4N4?
By Anonymous on Wednesday, April 21, 2004 - 12:25 am:
What should work is a cation exchange method. Luna SCX column with a mobile phase of KH2PO4 pH 2.5 with ACN or MeOH modifier. The compound will elute more quickly with increasing buffer strength ( could try say 20 to 50mM)
By Alexander on Friday, April 23, 2004 - 02:57 pm:
Try using Synergy C18 column. This column is notorious for very polar compounds enabling retention in very low ACN concentrations.
By huang on Sunday, April 25, 2004 - 05:40 am:
Thanks of all.My company have not these two kinds of columns,I will buy these,but I want to ask which company the Synergy C18 belong to?and I analysis the sample at CN column with the mobile phase(ACN:WATER 2:98),and the retention time is about 5 min, how do you think about it?
By AllsepTech on Sunday, April 25, 2004 - 11:10 am:
If you are going to buy new column consider Primesep. It allows you to retain amines, amidines, and aminoacids without ion-pairing reagents with higher organic concentration than 5%. Mobile phase is ACN-water-TFA (or formic, acidic acids, ammonium formate and ammonium acetate). Check this links:
http://www.allsep.com/makeChr.php?chr=Chr_017
http://www.allsep.com/makeChr.php?chr=Chr_041
http://www.allsep.com/makeChr.php?chr=Chr_012
Contact us if you need more information or want us to develop method for you.