By Charles on Tuesday, May 4, 2004 - 08:28 pm:
Hi,
I'm a student, curently on a research of validating method of simultaneous determination of three active ingredient. I have a trouble with one of them, the dextromethorphan Hbr, it doesn't give time retention even after 25 minutes (curent condition: HPLC varian, 4.6x250mm C-18, mobile phase of methanol:water=30:70 (v/v), 50:50(v/v),UV detection at 270nm)
After several trials, it was undetected. What I wish to ask is, does anybody know a known condition which give some result?
Buffer solution (phosphate buffer, acetate buffer, etc.) that I can use, before I have to use Ion-pairing agents. Thank you very much.
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By Anonymous on Wednesday, May 5, 2004 - 05:09 am:
Determination of dextromethorphan hydrobromide in bulk form and dosage formulations by high performance liquid chromatography
Abdel-Moety, E. M.; et al
J. Liq. Chromatogr.
1995, 18(20), Page: 4127