Did anyone have an expirience in determing response factor of olefins by np-hplc? Which parameters were used? Thanks for an answer!
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By Anonymous on Friday, May 7, 2004 - 03:28 pm:
Have never run olefins NP, but a common approach is to run several solutions at varying concs. of analytes and standard. Plot area vs. conc.; RF is ratio of slope of lines