By Anonymous on Friday, May 7, 2004 - 06:07 am:
My question is: I have to analyse DNA and in my samples are Oligonucleoties and perhaps DNA as residues of an digestion I only want to analyse Nucleotides and I have a quite good method for this but (Column: LiChroCART 250-4 RP18 Solvent:
50mM KH2PO4 pH4,1 2,5%ACN 1%MeOH 35°C)the remaining Oligonucleotides elute late and disturbe the following analyses
Which solvent can wash them out fast and quantitative or can I inject a solvent which washes tehm out
By Anonymous on Tuesday, May 11, 2004 - 06:09 am:
It`s me again would it be a benifit to injekt a 1M NaCl solution to wash the DNA residues out
By Anonymous on Thursday, May 13, 2004 - 01:18 am: