By Ravi Talluri on Monday, May 10, 2004 - 09:04 pm:
hello
I want to detect ascorbic acid and its oxidised product dehydroascorbic acid simultaneously by using hplc.please give me some suggestions in setting the method for it
By Uwe Neue on Tuesday, May 11, 2004 - 03:24 pm:
Look at the following website - many methods for ascorbic acid:
www.waters.com/WatersDivision/pdfs/catalog/Eappnotebook/Compd_Index_A.html
By Anonymous on Monday, May 17, 2004 - 06:24 am:
Forget about reverse phase, try some amino column with buffer.
Use Dithiothreitol to reduce the dehydroascorbic to ascorbic acid.