hi, there,

I have one question about Agilent 1100 Standard Autosampler. When I read the instruction about the injection, there is one item "Draw Position". In its definition, it said " The draw position is a vertical offset that enables you to position the needle a specific distance (in mm) away from its standard position.

So what is this "Standard Position"???? Is this the position when the needle first touches the vial location?

Thanks so much!!!

Anonymous,

I think that the manual refers to the buttom of the vial, at least that is my guess.

Kostas

Hi,
I believe it is the standard depth that the needle enters the vial. You may want to raise this slightly if you have only a small amount of sample available and are using a vial with an insert,to avoid hitting the bottom of the vial.

Keith.

The standard position is approximately 2 mm from the bottom of a normal HPLC vial. If you are using thicker bottomed vials, or set the value too low, the needle may hit it, which could result in damage to the needle, autosampler or vial. You can set a higher value to avoid picking anything up off the bottom of the vial, but this reduces the volume that you can inject.

I think Tim is right, it should be approximate 2mm from the bottom of a normal HPLC vial. Because there is one item in the manual also mentioned about "Limits"

Standard autosampler: -2.5 to 35 mm relative to the standard position

u autosampler: -2.5 to 35mm relative to the standard position.

If it is exactly the bottom of the vial, it could not have negative value.

Thanks so much for everyone for your response,

I believe the bottom of the vial or a plate is predefined from the "Preconfigured wellplate" or "User configured wellplate", not "a normal HPLC vial". All the dimentions are put into the software, and system use this parameters to define where is the bottom of the vial/plate.

jin