Methoxy phosphonic acid sodium salt
Chromatography Forum: LC Archives: Methoxy phosphonic acid sodium salt
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Labo on Wednesday, May 19, 2004 - 02:03 am:

I am currently trying to purify the title compound using HPLC method.
I have tried a SAX or NH2 staionnary phases but since I can't use acidic eluent, the compound always elute with the solvent front.

Does anyone has a solution to solve this problem?

Thanks

Top of pagePrevious messageNext messageBottom of pageLink to this message  By Uwe Neue on Wednesday, May 19, 2004 - 05:01 pm:

This is a very polar compound, and I would use HILIC on a HILIC silica column. Run a gradient from 95% acetonitrile to 50%, and then finetune the method.

Top of pagePrevious messageNext messageBottom of pageLink to this message  By Chris Pohl on Sunday, May 23, 2004 - 08:20 pm:

You should get retention of this compound at pH 5 or 6 with this compound via SAX. What eluent did you use? Try a weak eluent like acetate. How are you detecting this compound? It shouldn't absorb in the UV. You should be using conductivity detection for this compound.

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