Lower limits of detection of acid small compounds with 4.6mm columns

Chromatography Forum: LC Archives: Lower limits of detection of acid small compounds with 4.6mm columns
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Monday, May 24, 2004 - 07:33 am:

I would like to know if anybody has some data in
LOD of small acids. I get a LOD of 200 nanograms
per column, does it make sense?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Chris Pohl on Monday, May 24, 2004 - 08:20 pm:

That depends on the analyte, column dimensions and detector. What are your conditions?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, May 25, 2004 - 06:03 am:

For the LOD the compund used is 2,4-dichlorophenoxyacetic acid, the column lenght is 15 cm, I.D. is 4.6 mm and detector is a UV detector at 210 nm. Hope it's enough


Top of pagePrevious messageNext messageBottom of pageLink to this message  By bill tindall on Tuesday, May 25, 2004 - 09:41 am:

It seems way to high. I don't feel up to the conversion this AM but you can make it...........I have easily detected 1 ppm acetic acid with a 20 uL injection on 4.6 mm column. Acetic acid has an extinction coefficient more than an order of magnitude less than the acid you are dealing with.


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