By tom jupille on Sunday, May 30, 2004 - 04:51 pm:
Have you checked with the column manufacturer? Generally they are in the best position to make suggestions based on their knowledge of the material and input from other customers.
By Vida Ro on Wednesday, May 26, 2004 - 01:59 am:
I use a SEC Column in order to separate humic substances and their Cu complex! After 100-200 injections, separation problems appeared. Lack of resolution and different retention times. Humic substances are hydrophilic and soluble in alkalic solutions and my column is packed of a polymeric and hydrophilic material. I would be pleased if someone could propose me a regeneration procedure for these column and substance conditions!! Thank you