Does anyone have a methodology for Benzyl Benzoate soap? I developed one with Acetonitrile and Phosphoric Acid 0,1%, however I can't validate it. Precision results are totally weird. I don't know if it's because,to solve the soap, I let it in a hot bath.
Can anyone help me?

Dear Anon,
If your soap is fairly alakline I would worry about possible hydrolysis of the benzyl benzoate especially if you are heatiing it to help dissolve it. Maybe there is a way to dissolve it without heating it?

Regards,
Mark

I have only looked at benzyl benzoate once, and I used ACN with acetic acid-water on RP-18 at 280nm, similar to what you're using. Mark is likely on the right track, if you're talking about bar soap, which usually has pH about 9.8 to 10.1, because the heat and pH will cleave your ester linkages quickly. You'll also lose some benzyl benzoate over time at that pH, so your bar would need to be superfatted substantially to lower the pH to stabilize this. We rarely use water or heated water to dissolve bar soap samples. I would personally blend chopped bar sample with DMF, filter, and inject the DMF solution into the HPLC. Keep it simple.

Dear Mark and Consumer guy

What would my life be without you?

Thanks for the tip. Maybe DMF can help. Let's go again with all that development stuff. Me, that was almost validating it. heheh

Thanks again.