By Anonymous on Wednesday, June 9, 2004 - 10:06 am:
Trying to determine the % of SA after 60 days @ 45C.
I think my extraction using Methanol is creating Methyl
Salicylate which is lowering the % of SA that I would
expect.
Any thoughts ?
Thanks
By Consumer Products Guy on Wednesday, June 9, 2004 - 11:28 am:
When we formerly did salicylic acid assays, we extracted using methanol containing a few percent acetic acid. Chromatography was straightforward using water containing 4% acetic acid and either methanol or acetonitrile on RP-18. I don't think the methanol in the extraction solvent is causing your problem: if so, your 60 day ambient or 4C samples and your time zero samples would be affected the same way.
By Rjagielski on Wednesday, August 25, 2004 - 11:54 am:
Usually fisher esterification favors strongly acidic anhydrous conditons, in fact in the presence of a greater concentration of water for an extended amount of time any methyl salicylate would be hydrolized to salicylic acid.
An easy test... Smell it..