By Anonymous on Tuesday, June 15, 2004 - 04:55 am:
hello everybody
we are doing amino acid analysis by HPLC after OPA/Fmoc derivatization (similar to Aminoquant)
do you know of a nice method to end up the system during week-ends or after a day sequence ?
thanks
By Anonymous on Wednesday, June 16, 2004 - 01:24 am:
Do you mean to stop the machine?
I used to create a programe of finish with zero mL/min flow. Then, you programe another secuence with no sample.
If it's not what you ask, please tell me.
By Anonymous on Tuesday, June 22, 2004 - 12:53 am:
not to stop the machine or the pump, the Agilent software has a function to do this
I am looking for a method to rinse the column and store it
thanks
By RH on Friday, June 25, 2004 - 01:39 pm:
You should wash out the buffer salts by flushing
the column with a mixture of your organic
modifier and pure water (e.g. 50:50) with at
least 10 column volumes. Then you can run the
system with very low flow (0,02 ml/min) during
weekend.