By David on Monday, November 1, 1999 - 02:57 pm:
Does anyone have any insight to working with lutein or zeaxanthin in HPLC? Further, do any plant extractions exist using SPE?
By Anonymous on Monday, November 1, 1999 - 06:26 pm:
Usually reverse phase (C-18) columns work well : there are tons of methods for carotenoid HPLC (this was my MS work), so check out references by Khachik (especially good), Sadler&Tan, Krinsky. You should have no problem. Usual mobile phases include CH2Cl2 and THF. If you need any more help on this, I can detail it for you : adl120@psu.edu.
Good luck.
p.s. SPE extraction can be used, but solvent extraction is very easy and fast.
By Anonymous on Tuesday, November 2, 1999 - 05:52 am:
There's a paper by H. Stransky, published in Z. Naturforsch 33c (1978) pages 836 - 840 which details a method for the separation of plant pigments.
The paper summary reads as follows :
"A highly sensitive method is described for the determination of pigments in chloroplasts by HPLC. Less than one picomole of individual pigments - equivalent to 1mg fresh weight of spinach leaves - is needed for quantitation. Analysis time is 15 minutes, and pigments are protected against decomposition by light and air. Using isocratic elution, no equilibrium of columns is necessary, thus permitting four runs per hour."
By Anonymous on Monday, November 29, 1999 - 05:57 am:
AQUASIL C18,5 um, 250x4.6mm (Keystone Scientific,814-353-2300) gives baseline separation of lutein and zeaxanthin in about 10 minutes under nonaqusous reversed phase conditions. Mobile phase is 83%ACN/16% Dioxane/1% MeOH. Flow is 1.5 mL/min and UV detection is at 450 nm. For plant or plasma extracts guard columns packed with AQUASIL C18 should be used for extended column lifetimes. For more information contact Keystone Scientific tech support at 1-800-437-2999. A complete technical bulletin on vitamin analyis is also available
Posting is currently disabled in this topic. Contact your discussion moderator for more information.