Dear Friend!
i am working on identified the active material in root exudate that attract kind of worm, i did several tests and know i want to do one of the separation method. ( according to my tests my active material is cation charge, is adsorption to RF colona is not destroid in High Temp )
which kind of separation method i have to use?
Thank you very much for your time.
I appreciate it allot.
Ziv

Ziv,

Put your mixture on a big TLC plate and run it in a polar system. Dry it down at room temperature not to degrade the attractant and let the worms to crawl to the plate. Carefully check with a pencil the area that the worms crowded, cut the band and isolate the attractant.

I do it all the time with my mosquitoes. Always works!