By Tristan on Sunday, July 4, 2004 - 08:46 am:
G'day all,
I am relatively new to liquid chromatography and only have experience with RP-HPLC. I have noticed in a few conversations that HILIC is mentioned/recommended. In what situations is HILIC best? Can someone please suggest some references or reading material on this subject?
Thanks all!!
By Chris Pohl on Saturday, July 10, 2004 - 02:33 pm:
HILIC is just another name for normal phase chromatography (it's supposed to stand for: Hydrophilic Interaction Chromatography). The acronym for this should have been HIC but this acronym was already taken for another technique: Hydrophobic Interaction Chromatography (previously known as Salting Out Chromatography). There are quite a number of references on normal phase chromatography but I would recommend you start with Snyder and Kirkland's book on HPLC.
By Einar Pontén - SeQuant AB on Sunday, July 11, 2004 - 02:12 am:
I don't agree! HILIC does mean something else than "normal phase".
In HILIC you use a hydrophilic stationary phase (as in normal phase) and a water soluble mobile phase (as in reversed phase). That offers you a tool to retain very hydrophilic and water soluble compounds, like carbohydrates, amines, alcohols, acids.
A recent paper is:
"Determination of neomycin by LC-tandem mass spectrometry using hydrophilic interaction chromatography"
Oertel, R., Renner, U., Kirch, W.,
J Pharm Biomed Anal 35(3) 633-8, 2004
By tom jupille on Monday, July 12, 2004 - 09:55 am:
But the fact is that the term "normal phase chromatography" has been applied to the type of system you describe for *at least* the past 20 years (carbohydrates using amino bonded-phase columns with water/acetonitrile mobile phase). If tagging a useful technique with a new name helps to popularize it, fine, but we should at least recognize the relationship (IMHO).
How about just recognizing HILIC as a subset or special case of normal phase?
By Einar Pontén - SeQuant AB on Monday, July 12, 2004 - 03:20 pm:
OK, you are correct there are not that sharp borders between techniques. However, for a lot of chemists and a long time "normal phase" has been identified as a technique where solvents immicible with water are most common. HILIC is based on the use of solvents commonly used in reversed phase.
By they way, "HIC" mentioned above is better described as "salting out chromatography" although the new name might have popularized it...