Hi,I am using Prep HPLC- Facing PROBLEMS .Can any body suggest the solution for Aqueous 0.1% TFA with ACN (50:50)created air lock in Prep HPLC
Chromatography Forum: LC Archives: Hi,I am using Prep HPLC- Facing PROBLEMS .Can any body suggest the solution for Aqueous 0.1% TFA with ACN (50:50)created air lock in Prep HPLC
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Mike on Wednesday, July 14, 2004 - 03:06 am:

Hi,I am using Prep HPLC- Facing PROBLEMS .Can any body suggest the solution for Aqueous 0.1% TFA with ACN (50:50)created air lock in Prep HPLC

Top of pagePrevious messageNext messageBottom of pageLink to this message  By henrik on Sunday, August 1, 2004 - 04:35 am:

Do you degass you mobilephase before use?

Top of pagePrevious messageNext messageBottom of pageLink to this message  By A.Mouse on Sunday, August 1, 2004 - 08:13 am:

Is the solvent bottle lower than the inlet of the pump? Do you use filters in the lines from solvent container to pump?

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