By Andy Minkevich on Wednesday, July 21, 2004 - 10:10 am:
I would appreciate feedback on how I can improve the resolution of my monomer and multimer peaks using size exclusion.
By GPC user on Wednesday, July 21, 2004 - 02:44 pm:
Currently do you have any columns? If so, what are their molecular range covered?
By Chris Pohl on Sunday, July 25, 2004 - 03:51 pm:
Andy,
What degree of polymerization (DP) are you dealing with? You might be able to separate the oligimers from one another and from the monomer by reversed phase if the DP isn't too high.
By srinivas kone on Monday, July 26, 2004 - 03:45 am:
You pl. try in GFC systems using phosphate buffer with SDS, if your sample is water soluble and use columns in serial , you may get better resolution