Has anyone tried to determine low levels of Teepol by HPLC? - I need to be able to select amongst other detergents for the purposes of a cleaning verification at around <150ug/dm2

I've injected Teepol down a Hypersil ODS 5 micron column. It was part of an investigation into spurious peaks in a related substances bulk drug assay.

There were peaks with pH 4.5 (sodium acetate buffer) / acetonitrile - but whether you'd be able to separate the Teepol from other detergents is another matter.

I got more help from the column manufacturer (Hypersil......or ThermoQuest Chromatography Supplies as it is now). They had applications for ionic and non-ionic surfactants.

I'm in the UK, but I believe there's a US office if you're in that area. There's also a web site at www.hypersil.com

Thanks for the tip - did you come up with a method of detection - UV or other?

I still have the same problem.

t.

There is literature regarding analysis of alkanesulfonate and alkyl sulfate surfactant with detection limit 0.3 nM. ( I am sorry that I could not figure out what is ug/dm2). It showed base line separate C2 to C18 surfactants. If you are interest in the literature, please let me know you fax number.

Best regards,

Thankyou for your help-I still have the problem ! - If you would kindly fax me a copy of the lit to 44(UK) (0)1229 482257, I would be most greatful.

We tend to express a contamination as a function of the surface area of an item such as a vessel etc hence - microgram's per decM 2(10cm x 10cm)

many thanks

t.

Dear Toni,
I already faxed over the literature you are interest in. Please check it. Good luck.

Best regards,

kindly send me the hplc method of analysis of teepol