By Meifi on Wednesday, July 28, 2004 - 05:54 pm:
Hello...
I need suggestion of chromatgraphy system for risendronate determination. We have used ion pairing chromatography with 0.005 M Tetrabutyl amoniumhydrogen sulfate, but the peak is fat.
Anyhelp will be appreciated.
By Chris Pohl on Friday, July 30, 2004 - 01:10 am:
Your problem is most likely due to the fact that this compound is a decent chelating agent and metal contamination of your sample, column and/or eluent is responsible for the tailing due to partial separation of the complexed and "naked" forms of the chelator (along with on column interconversion of the two forms). See my comments in another recent thread "Mobile Phase Additive for Chelating Agent" for suggested corrective actions.