We are currently using an organic acid column to quantitate chloride (a very large peak!), dextrose(a smaller peak that rides the tail of the chloride peak), and acetic acid in kidney dialysate. My problem is: how can I get complete separation of the chloride and dextrose peaks? By the way, I'm using uv/vis detection at 193 nm and .005 M H2SO4 mobile phase.

I am guessing that you are using a sulfonated styrene-divinylbenzene column. What temperature are you running at? If you have a column heater, try varying the temperature of the column. You may get significant improvement. Consult the manufactures recommendations on proper care and feeding of theses columns for the maximum temperature. You can increase you plate number (and resolution) to some extent by increasing the concentration of H2SO4 in your mobile phase. Try 0.01 and 0.015 M. Again, I would check the column specifications on the concentration of H2SO4 you should not to exceed. The maximum concentration of H2SO4 may be dictated by your sample more so than your column. These columns are quite rugged.

Please be kind enough to post how things worked out for you.

Is a conductivity detector an option? By using
conductivity for peak detection you dont have to
worry about dextrose at all.