By Teresa Chong on Tuesday, November 16, 1999 - 05:20 am:
can anyone please provide the procedures for analysing sodium acetate? What kind of column should be selected? What kind of mobile phase should be used?
By B.Buglio on Saturday, November 27, 1999 - 08:51 am:
Suggest converting to the acid and using either
ion suppression or ion exclusion chromatography.
With the fomer use an 0.1 M KH2PO4,pH2 mobile
phase and a C8 column. With the latter, an 0.005N
H2SO4 mp and a cation exchange column. Monitor at
220nm in either ca
By Anonymous on Tuesday, November 30, 1999 - 12:20 pm:
The acid will be very polar and probably won't retained on a conventional alkyl C8 column. A polar bonded phase like AQUASIL C18 (Keystone Scientific, 1-800-437-2999) under 100% aquous conditions (0.05M KH2PO4, pH 2) should give adequate retention.
AQUASIL C18 is a silica-based packing designed with a high concentration of C18 groups, while preserving hydrophilic sites on the surface to assist in retention and resolution of highly water-soluble compounds, such as nucleotides, and organic acids. The C18 chains remain extended for maximum retention stability even in pure aqueous mobile phases.
For more information contact Keystone Scientific tech support.
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