By Eric DeSimone on Sunday, November 21, 1999 - 02:18 pm:
I' having trouble in finding the concentration of a molecule given the area of a given peak from the HPLC.
I also don't know how the graph is set up.
By Anonymous on Monday, November 22, 1999 - 05:25 am:
First day with your LC?
Run standards of known concentrations, plot them on a graph (conc. vs area). Compare the area of your unknown to the graph, calculate result. Advanced students will calculate the equation of the line and use that formula to calculate the concentration of the unknown.
By Anonymous on Monday, November 22, 1999 - 06:03 am:
Or just make the graph in Excel/Lotus/Quattro Pro, and let the data regression calculate the slope & intercept. Then calculate the concentration using that good ole y=mX+b equation.
By Vansuela on Monday, November 22, 1999 - 09:29 am:
If you are using a single point calibration, calculate the response factor as conc (std)/Area (std) and then for the unknown just divide the area by the response factor.
Or if you are calibrating using a curve, basically its going to be the same Math
if your curve passes through the origin (y=mX), calculate X=y/m and if not calculate X=y-C/m, if the curve does not pass through the origin!
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