I work at laboratory which often has to run gradient methods developed in other laboratories. Some of the methods we take over are quite complicated and not robust at all. Many times there are endless discussions about volumes (wrong measures) or detectors and other differences between systems. I believe that I have read an article about the transferability of gradient methods but I dont remember where I read it. I remember that authors had a discussion about the volumes between the pump and the column (not between the autosampler and the column. Can anyone provide me wíth some good references?

Check pages 386-392 of the book "Practical HPLC Method Development (2nd ed) by Snyder, Glajch, and Kirkland. Published by Wiley (you can buy it from LC Resources, hint, hint). Another good thorough discussion is in and article by Snyder and Dolan, LC/GC 8(7), 524 (1990).

The help files for our DryLab software also have a lot of information about dwell volume, You can also download a free evaluation copy of at http://www.lcresources.com/dlmain.htm. Look in the help file index under the topic "dwell volume" for information. The program itself lets you model what a separation will look like on an instrument with a different dwell volume (or extra-column volume, for that matter).

Hope this helps.

-- Tom Jupille / LC Resources

HI
Check u'r instrument !
When u using Gradient delay function, you can transfer u'r gradient method to other labs.
You need a autosampler included gradient delay.
I using TSP AS3000 to run gradient delay.