By Anonymous on Saturday, December 18, 1999 - 12:15 pm:
How the two methods for the determination of LOD-LOQ (i)S/N ratio and (ii)Standard error of Y estimate(Residual standard deviation) are equivalent?
By Adrian on Monday, December 20, 1999 - 04:28 am:
They both try to estimate the area of your assay range which will introduce detection variability.
The signal to noise ratio tries to determine this point of variability using one single determination while the y-intercept approach uses the lower range, leading into the loQ and further into the LoD of your assay.
Another approach which seems to have a more practical and scientific rationale is the EURACHEM approach to determining LoQ/Lod. This involves injecting a decreasing range of standards 6 times,
and ploting conc vs. %RSD. By extrapolating the predetermined %RSD for your assay you can determine an LoQ and also an LoD if required.
By Maciej on Thursday, January 6, 2000 - 08:39 pm:
Yes, your're right. Sorry, I think of C18 automatically, as it is fundamental column to me.
Greets,
Maciej
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