According my colleague, the terms of High Load and Low Load are about HPLC method. Low Load is used for a potency indicate HPLC method. A very dilute solution is injected to the column to avoid detector saturation and column overload. A high solution is used for degradant and impurity indication method so much more sample can be loaded to measure trace amount of degradants or impurities. So far, the theory is sound. However, he believes that if a HPLC method applies both potency and stability (impurity, degradants), the method must be not following the Beer’s Law. The reason, according to him, a trace component, say A, and the major component, say B, can not measured at the same method. If both A and B measured with the same method, the major component must be saturated regardless both components have a linearity R2>0.99. Thus, according him, there should be two HPLC methods, one for potency, one for stability. Does this sound right to you. Are most of the industries using two methods for potency and stability determinations? Please tell me know what you think. Thanks.